Dr. Juan B. Ochoa, MD:  Publications and Grants
Publications:

1.  Makarenkova VP, Bansal V, Matta BM, Perez LA, Ochoa JB. CD11b+/Gr-1+ myeloid suppressor
cells cause T cell dysfunction after traumatic stress. J Immunol. 2006 Feb 15;176(4):2085-94.

2.  Harbrecht BG, Zenati MS, Alarcon LH, Ochoa JB, Puyana JC, Schuchert VD,
Peitzman AB. Is outcome after blunt splenic injury in adults better in high-volume trauma centers? Am
Surg. 2005 Nov;71(11):942-8; discussion 948-9.

3.  Ochoa JB, Makarenkova V. T lymphocytes. Crit Care Med. 2005 Dec;33(12 Suppl):S510-3. Review.
No abstract available.

4.  Ochoa JB, Makarenkova V, Bansal V. A rational use of immune enhancing diets: when should we
use dietary arginine supplementation? Nutr Clin Pract. 2004 Jun;19(3):216-25.

5.  Tsuei BJ, Magnuson B, Swintosky M, Flynn J, Boulanger BR, Ochoa JB, Kearney
PA.  Enteral nutrition in patients with an open peritoneal cavity. Nutr Clin Pract. 2003 Jun;18(3):253-8.

6.  Rodriguez PC, Hernandez CP, Quiceno D, Dubinett SM, Zabaleta J, Ochoa JB,
Gilbert J, Ochoa AC.  Arginase I in myeloid suppressor cells is induced by COX-2 in lung carcinoma. J
Exp Med. 2005 Oct 3;202(7):931-9.

7.  Zea AH, Rodriguez PC, Culotta KS, Hernandez CP, DeSalvo J, Ochoa JB, Park HJ, Zabaleta J,
Ochoa AC.  L-Arginine modulates CD3zeta expression and T cell function in activated human T
lymphocytes. Cell Immunol. 2004 Nov-Dec;232(1-2):21-31.]

8.  Bansal V, Syres KM, Makarenkova V, Brannon R, Matta B, Harbrecht BG, Ochoa JB. Interactions
between fatty acids and arginine metabolism: implications for the design of immune-enhancing diets.
JPEN J Parenter Enteral Nutr. 2005 Jan-Feb;29(1  Suppl):S75-80. Review.

9.  Bansal V, Rodriguez P, Wu G, Eichler DC, Zabaleta J, Taheri F, Ochoa JB. Citrulline can preserve
proliferation and prevent the loss of CD3 zeta chain under conditions of low arginine. JPEN J Parenter
Enteral Nutr. 2004 Nov-Dec;28(6):423-30.

10.  Rodriguez PC, Quiceno DG, Zabaleta J, Ortiz B, Zea AH, Piazuelo MB, Delgado A, Correa P, Brayer
J, Sotomayor EM, Antonia S, Ochoa JB, Ochoa AC.  Arginase I production in the tumor
microenvironment by mature myeloid cells inhibits T-cell receptor expression and antigen-specific T-cell
responses. Cancer Res. 2004 Aug 15;64(16):5839-49.

11.  Marderstein EL, Simmons RL, Ochoa JB. Patient safety: effect of institutional protocols on adverse
events related to feeding tube placement in the critically ill. J Am Coll Surg. 2004 Jul;199(1):39-47;
discussion 47-50.

12.  Barksdale AR, Bernard AC, Maley ME, Gellin GL, Kearney PA, Boulanger BR, Tsuei BJ, Ochoa JB.
Regulation of arginase expression by T-helper II cytokines and isoproterenol. Surgery. 2004
May;135(5):527-35.

13.  Harbrecht BG, Zenati MS, Ochoa JB, Townsend RN, Puyana JC, Wilson MA, Peitzman AB.
Management of adult blunt splenic injuries: comparison between level I and level II trauma centers.
J Am Coll Surg. 2004 Feb;198(2):232-9.

14.  Rodriguez PC, Zea AH, DeSalvo J, Culotta KS, Zabaleta J, Quiceno DG, Ochoa JB, Ochoa AC.
L-arginine consumption by macrophages modulates the expression of CD3 zeta chain in T lymphocytes.
J Immunol. 2003 Aug 1;171(3):1232-9.

15.  Bansal V, Ochoa JB. Arginine availability, arginase, and the immune response. Curr Opin Clin Nutr
Metab Care. 2003 Mar;6(2):223-8. Review.

16.  Ochoa JB, Ford HR. Invited commentary: new roles for immune-enhancing diets. Surgery. 2002
Nov;132(5):815-6. No abstract available.

17.  Rodriguez PC, Zea AH, Culotta KS, Zabaleta J, Ochoa JB, Ochoa AC. Regulation of T cell receptor
CD3zeta chain expression by L-arginine. J Biol Chem. 2002 Jun 14;277(24):21123-9.

18:  Harbrecht BG, Peitzman AB, Rivera L, Heil B, Croce M, Morris JA Jr, Enderson BL, Kurek S,
Pasquale M, Frykberg ER, Minei JP, Meredith JW, Young J, Kealey GP, Ross S, Luchette FA, McCarthy
M, Davis F 3rd, Shatz D, Tinkoff G, Block EF, Cone JB, Jones LM, Chalifoux T, Federle MB, Clancy KD,
Ochoa JB, Fakhry SM, Townsend R, Bell RM, Weireter L, Shapiro MB, Rogers F, Dunham CM,
McAuley CE.  Contribution of age and gender to outcome of blunt splenic injury in adults: multicenter
study of the eastern association for the surgery of trauma. J Trauma. 2001 Nov;51(5):887-95.

19.  Tsuei BJ, Bernard AC, Shane MD, Shirley LA, Maley ME, Boulanger BR, Kearney PA, Ochoa JB.
Surgery induces human mononuclear cell arginase I expression. J Trauma. 2001 Sep;51(3):497-502.

20.  Boulanger BR, Kearney PA, Tsuei B, Ochoa JB. The routine use of sonography in penetrating
torso injury is beneficial. J Trauma. 2001 Aug;51(2):320-5.  

21.  Taheri F, Ochoa JB, Faghiri Z, Culotta K, Park HJ, Lan MS, Zea AH, Ochoa AC. L-Arginine
regulates the expression of the T-cell receptor zeta chain (CD3zeta) in Jurkat cells. Clin Cancer Res.
2001 Mar;7(3 Suppl):958s-965s.

22.  Bernard AC, Mistry SK, Morris SM Jr, O'Brien WE, Tsuei BJ, Maley ME, Shirley LA, Kearney PA,
Boulanger BR, Ochoa JB.  Alterations in arginine metabolic enzymes in trauma. Shock. 2001
Mar;15(3):215-9.

23.  Ochoa JB, Bernard AC, O'Brien WE, Griffen MM, Maley ME, Rockich AK, Tsuei BJ, Boulanger BR,
Kearney PA, Morris Jr SM Jr. Arginase I expression and activity in human mononuclear cells after injury.
Ann Surg. 2001 Mar;233(3):393-9.

24.  Ochoa JB, Strange J, Kearney P, Gellin G, Endean E, Fitzpatrick E. Effects of L-arginine on the
proliferation of T lymphocyte subpopulations. JPEN J Parenter Enteral Nutr. 2001 Jan-Feb;25(1):23-9.

25.  Peitzman AB, Heil B, Rivera L, Federle MB, Harbrecht BG, Clancy KD, Croce M, Enderson BL,
Morris JA, Shatz D, Meredith JW, Ochoa JB, Fakhry SM, Cushman JG, Minei JP, McCarthy M, Luchette
FA, Townsend R, Tinkoff G, Block EF, Ross S, Frykberg ER, Bell RM, Davis F 3rd, Weireter L, Shapiro
MB. Blunt splenic injury in adults: Multi-institutional Study of the Eastern Association for the Surgery of
Trauma. J Trauma. 2000 Aug;49(2):177-87; discussion 187-9.

26.  Ochoa JB, Bernard AC, Mistry SK, Morris SM Jr, Figert PL, Maley ME, Tsuei BJ, Boulanger BR,
Kearney PA. Trauma increases extrahepatic arginase activity. Surgery. 2000 Apr;127(4):419-26. PMID:
10776433

27.  Bernard AC, Fitzpatrick EA, Maley ME, Gellin GL, Tsuei BJ, Arden WA, Boulanger BR, Kearney PA,
Ochoa JB. Beta adrenoceptor regulation of macrophage arginase activity. Surgery. 2000
Apr;127(4):412-8.

28.  Kearney PA, Griffen MM, Ochoa JB, Boulanger BR, Tseui BJ, Mentzer RM Jr. A single-center
8-year experience with percutaneous dilational tracheostomy. Ann Surg. 2000 May;231(5):701-9.

29.  Broto J, Asensio M, Jorro CS, Marhuenda C, Vernet JM, Acosta D, Ochoa JB. Conservative
treatment of caustic esophageal injuries in children: 20 years of experience. Pediatr Surg Int. 1999
Jul;15(5-6):323-5.

30:  Jacob TD, Ochoa JB, Udekwu AO, Wilkinson J, Murray T, Billiar TR, Simmons RL, Marion DW,
Peitzman AB. Nitric oxide production is inhibited in trauma patients. J Trauma. 1993 Oct;35(4):590-6;
discussion 596-7.

31.  Harbrecht BG, Billiar TR, Stadler J, Demetris AJ, Ochoa JB, Curran RD, Simmons RL. Nitric oxide
synthesis serves to reduce hepatic damage during acute murine endotoxemia. Crit Care Med. 1992
Nov;20(11):1568-74.

32.  Ochoa JB, Curti B, Peitzman AB, Simmons RL, Billiar TR, Hoffman R, Rault R, Longo DL, Urba WJ,
Ochoa AC. Increased circulating nitrogen oxides after human tumor immunotherapy: correlation with
toxic hemodynamic changes. J Natl Cancer Inst. 1992 Jun 3;84(11):864-7. Erratum in: J Natl Cancer Inst
1992 Aug 19;84(16):1291.

33.  Ligush J Jr, Labadie RF, Berceli SA, Ochoa JB, Borovetz HS. Evaluation of endothelium-derived
nitric oxide mediated vasodilation utilizing ex vivo perfusion of an intact vessel. J Surg Res. 1992
May;52(5):416-21.

34.  Billiar TR, Curran RD, Harbrecht BG, Stadler J, Williams DL, Ochoa JB, Di Silvio M, Simmons RL,
Murray SA. Association between synthesis and release of cGMP and nitric oxide biosynthesis by
hepatocytes. Am J Physiol. 1992 Apr;262(4 Pt 1):C1077-82.

35.  Langrehr JM, Dull KE, Ochoa JB, Billiar TR, Ildstad ST, Schraut WH, Simmons RL, Hoffman RA.
Evidence that nitric oxide production by in vivo allosensitized cells inhibits the development of
allospecific CTL. Transplantation. 1992 Mar;53(3):632-40.

36.  Ochoa JB, Udekwu AO, Billiar TR, Curran RD, Cerra FB, Simmons RL, Peitzman AB. Nitrogen oxide
levels in patients after trauma and during sepsis. Ann Surg. 1991 Nov;214(5):621-6.

37.  Billiar TR, Langrehr JM, Curran RD, Ochoa JB, Stadler J, Harbrecht BG, Hoffman RA, Simmons RL.
Two unique aspects of inducible .N = O synthase in liver cells and accessory cells: hepatic damage is
minimized by hepatocyte .N = O production and immunoregulation is mediated by macrophage .N = O
production. Res Immunol. 1991 Sep;142(7):584-6; discussion 598-9. Review.

38.  Stadler J, Billiar TR, Curran RD, Stuehr DJ, Ochoa JB, Simmons RL. Effect of exogenous and
endogenous nitric oxide on mitochondrial respiration of rat hepatocytes.Am J Physiol. 1991 May;260(5
Pt 1):C910-6.

39.  Loeffler CM, Platt JL, Anderson PM, Katsanis E, Ochoa JB, Urba WJ, Longo
DL, Leonard AS, Ochoa AC. Antitumor effects of interleukin 2 liposomes and anti-CD3-stimulated T-cells
against murine MCA-38 hepatic metastasis. Cancer Res. 1991 Apr 15;51(8):2127-32.

40.  Stadler J, Curran RD, Ochoa JB, Harbrecht BG, Hoffman RA, Simmons RL, Billiar TR. Effect of
endogenous nitric oxide on mitochondrial respiration of rat hepatocytes in vitro and in vivo. Arch Surg.
1991 Feb;126(2):186-91.

41.  Curran RD, Billiar TR, Stuehr DJ, Ochoa JB, Harbrecht BG, Flint SG, Simmons RL. Multiple
cytokines are required to induce hepatocyte nitric oxide production and inhibit total protein synthesis.
Ann Surg. 1990 Oct;212(4):462-9; discussion 470-1.

42.  Broto J, Ilari J, Moraga FA, Sarto J, Bertran JM, Ochoa JB, Enriquez G. [Bile peritonitis caused by
idiopathic perforation of a choledochal cyst] Arch Fr Pediatr. 1986 Dec;43(10):795-7. French.

43.  Ochoa JB. Diagnosis and management of gastroesophageal reflux in children. Surg Annu.
1981;13:123-37.

44.  Lucaya J, Ochoa JB. Ectopic pancreas in the stomach. J Pediatr Surg. 1976 Feb;11(1):101-2. No
abstract available.

45.  Ochoa JB, Gosalbez R. [Obstruction of the lower urinary tract] Minerva Pediatr. 1974 Sep
22;26(29):1451-79. Italian.


Grants:

RESEARCH SUPPORT (ongoing or completed within past 3 years):

RO1 GMO65914-01A2                 04/01/2005-03/31/2006        
NIH/                                               $252,138
Immature Myeloid Cells and T Cell Suppression in Trauma

K08-GM-00676 (Ochoa)               8/1/99-7/31/02      75%
NIH/NIGMS                                    $109,122
Causes and Effects of Arginase Activity After Trauma

M2003-0025                                 May, 2003
Pittsburgh Society Foundation      $150,000
Immunosuppression in cancer: A matter of malnutrition.

The aims of this project are:
1) Characterize the changes in tissue arginases caused by trauma;
2) Identify which factors are responsible for the increase in arginase activity after trauma;
3) Determine the effects of increased arginase by macrophages